MiCo Biomed USA – I use a conventional (absorbance) reader for my ELISA; I know that I will need a fluorescence reader but what other changes will I need for the ELISA itself?

I use a conventional (absorbance) reader for my ELISA; I know that I will need a fluorescence reader but what other changes will I need for the ELISA itself?

Nothing! Most absorbance (colorimetric) assays use an HRP label for the detection antibody to quantify assay signal. All you need is the OptiGlow™ substrate which uses the same HRP enzyme to generate a chemifluorescence signal.