MiCo Biomed USA – I have used clear-bottom 96-well plates for fluorescence detection but they have black sidewalls, or the plate is made of completely black material. Optimiser™ is completely clear – won’t it have a very high crosstalk between wells?

Actually – NO. There is no crosstalk between Optimiser™ wells. The reason conventional plates have a black sidewall or completely black material is to minimize the autofluorescence which leads to increased background noise. All fluorescence readers read one well at a time so there is no signal from the adjacent well (since the fluorescent entity in the adjacent well is not being excited). The tapered profile of the loading well also helps in directing the excitation light towards the channel underneath the well rather than to adjacent channels.

I have used clear-bottom 96-well plates for fluorescence detection but they have black sidewalls, or the plate is made of completely black material. Optimiser™ is completely clear – won’t it have a very high crosstalk between wells?