• Most binding reactions on Optimiser™ microplates saturate in ∼ 5 minutes. Users can actually use even 5 minute incubation steps (except for sample which should be at least 20 minutes). The Application Note section has an article that describes this in greater detail.
  • We recommend that you start with at least 10 minute incubation cycles/step – but you can certainly use longer (up to 20 minutes) incubation steps. This may be useful when you are processing multiple Optimiser™ plates in parallel.
  • All incubation steps must be at least 5 minutes (at least 20 minutes for sample). Incubation steps should not exceed 20 minutes.