• Most importantly – please check if your solutions are clear of particulates (e.g., supernatants from cell lysate).
  • We strongly recommend the use of OptiMax™ buffers with Optimiser™ microplates. OptiMax™ buffers are specifically developed for and validated for use with Optimiser™ microplates.
  • We have validated Optimiser™ plates with aqueous solutions only. Do not use solvent-containing samples.
  • Do not use reagents containing ANY surfactants with Optimiser™ microplates.
  • Very rarely (<0.2%) you will see liquid that has not drained out even after ∼ 10 minutes. This indicates a flow failure and signal from that well should not be used for analysis.