• In conventional 96-well ELISA plates, if the pipette tip touches the bottom surface of the well, it may physically disrupt some of the bound bio-molecules. In the Optimiser™ all the assay reactions occur within the microchannel. Hence, touching the pipette tip on the loading well of the Optimiser™ has absolutely no effect on the assay performance.
  • For most dispensing systems in Optimiser™ based assays, users are dispensing only 5 μl volumes. If the pipette tip does NOT touch the well surface, the dispensed well volume may “bead” and stick to the end of the tip. The well geometry of the Optimiser™ is engineered to ensure smooth filling of well/microchannel provided the liquid is dispensed steadily and directly on the well surface.
  • See the Optimser™ Technology page for instructional videos on pipetting techniques.